Biology has many topics in it which are demanding both time and commitment. This means although the subject is interesting to many learners a high percentage fail assignments and examinations. Contact My homework writers for Biology assignment help from our experts. The following question is a sample.
The substance N-glutaryl-L-phenylalanine-p-nitrophenol (GPN) can be used as a so-called chromogenic substrate for the protease chymotrypsin.
1. Describe what is meant by a “chromogenic substrate”, including which properties of GPN that allow it to be used as a substrate for chymotrypsin.
In the enzymatic reaction, the substance p-nitrophenol is formed, which is a weak acid with a pka- value of 7.15. Below are absorption curves for the two protonation forms of the substance.
2. Indicate in bullet points the experimental steps you want to take to set up a standard curve for the activity of chymotrypsin against GPN measured by spectroscopy. The standard curve should show initial velocity as a function of enzyme concentration. Specify selection of pH value and wavelength as well as suggestions for other components in the reaction solution.
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