Biology is a subject taught in many institutions because it equips the learners with different skills not only for career security but also for living in the environment. The subject has many topics which are interesting to learners. However, the one about enzymes seems confusing for students mainly when answering Biology questions. At my home work writers, we offer assistance in the subject area. The quest ion below has been questioned frequently.
An enzyme is labeled with two fluorophores that form a FRET pair with RO = 54 Å and analyzed with FRET spectroscopy. In the absence of ligand, a constant FRET efficiency of E = 0.2 is measured.
1. What is the distance between the two fluorophores?
After addition of the substrate, the following FRET efficiency (E) is observed as a function of time for a representative enzyme molecule. Below is the result of two experiments performed respectively with (black) and without (red) substrate.
2. Describe the effect of the substrate on the structure and dynamics of the enzyme from the above graph.
The dynamics of the enzyme can be interpreted from a model with two states, S1 and 52, where S1 has low and S2 has high FRET efficiency.
For a mutant of the enzyme, the following data are now available. FRET effectivity (E) MULUT 10 Time (5)
3. How does mutation (increase or decrease) affect k1, k-1, and Keq under the presence of substrate? Justify your answer.
4. For a wide variety of substrates, the enzyme has the same kcat. Explain this observation based on what you know about the dynamics of the enzyme.
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